DEPARTMENT OF APPLIED CHEMISTRY AND MATERIAL SCIENCE,

ENGINEERING FACULTY, UNIVERSITY OF BOLOGNA

Viale Risorgimento, 2, 40136 Bologna, Italy

 

Project Co-Director: Prof. Fabio Fava (NPD)

Telefon/Fax: (39 051) 2093212/  (39 051) 2093218

E-mail: fabio.fava@mail.ing.unibo.it

 

The Faculty of Engineering of the Bologna University (Appendix 1.5) was funded in 1936.Attended by more than 12 000 students, it groups 11 different Master Degree programs and 9 Undergraduate degree programs. The Department of Applied Chemistry and Material Sciences belongs to this Faculty, and it counts about 40 people, 25 of which are Researchers, Lecturers and Professors. The research activity of the Department is focused on 3 different fields: Polymer Chemistry, Materials Science and Technologies and Environmental Biotechnology.

 

The knowledge existing in the Prof. Fava’s group, BU team (the only one of the Department involved in environmental biotechnology researches), can be summarized as follows:

·        Study of biodegradation processes of PCBs, PAHs, chlorobenzoic acids and chlorophenols in aerobic conditions, and of PCBs in anaerobic conditions. In particular, this research activity included: I) isolation and taxonomic characterization of the bacteria involved in the biodegradation process, II) characterization of the biodegradative pathways through which each xenobiotic is degraded by chemical, biochemical and genetic tools and III) determination of the main kinetics parameters describing the biodegradation process of the xenobiotics.

·        Optimization of the PCB and chlorobenzoic acid aerobic biodegradation in synthetic wastewaters by: I) stimulating the microorganism growth and metabolic activity by addition of growth cofactors II) enhancing the xenobiotic bioavailability through addition of surfactants, cyclodextrins, or  physical  agents.

·        Development of aerobic lab-scale bioreactors (mainly packed-bed bioreactors with passively immobilized cells) for the batch and the continuous treatment of PCBs-  and chlorobenzoic acid-contaminated waste-waters  and for the biological treatment of olive oil mill wastewater.

·        Development of aerobic lab-scale bioreactors for the slurry-phase, the semisolid-phase and the fixed-phase  "ex-situ" treatment of actual site PCB- and PAH-contaminated soils. Attempts to improve the PCB contaminated soil bioremediation by adding exogenous specialized biomass as well as PCB-solubilizing agents, such as   cyclodextrins, synthetic and biogenic surfactants, and humic acid fractions, into the soil.

List of some recent and relevant publications

·        Fava F., Grassi F. (1996) Cyclodextrins enhance theaerobic degradation and dechlorination of low-chlorinated biphenyls Biotechnology Techniques, 10: 291-296.

·        Fava F., Baldoni F., Marchetti L., Quattroni G. (1996) A bioreactor system for the mineralization of low-chlorinated biphenyls Process Biochemistry 31: 659-667.

·        Fava F., Di Gioia D., Marchetti L., Quattroni G. (1996) Aerobic dechlorination of low-chlorinated biphenyls by bacterial biofilms in packed-bed bioreactors Applied  Microbiology and Biotechnology 45: 562-568.

·        Fava F., Di Gioia D., Marchetti L. (1996) Dichlorobiphenyl degradation by an uncharacterized Pseudomonas species,strain CPE1, in a fixed film bioreactor. International  Biodeterioration  & Biodegradation. 37: 53-59.

·        Pinelli D, Fava F., Nocentini M., Pasquali G. (1997).Bioremediation of a polycyclic aromatic hydrocarbon-contaminated soil by using different aerobic batch bioreactor systems Journal of Soil Contamination 6: 243-256.

·        Fava F., Di Gioia D., Marchetti L. (1998) Cyclodextrins effects on the ex-situ bioremediation of a chronically polychlorobiphenyl-contaminated soil Biotechnology & Bioengineering58: 345-355.

·        Di Gioia D., Peel M., Fava F., Wyndham C. (1998) Structure of homologous composite transposons carrying cba ABC genes from Europe and North America .Applied Environmental Microbiology, 64: 1940-1946.

·        Fava F., Di Gioia D. (1998) Effects of Triton X-100 and Quillaya Saponin on the ex-situ bioremediation of a chronically polychlorobiphenyl-contaminated soil Applied Microbiology and Biotechnology, 50: 623-630.

·        Fava F., Bertin L. (1999) Influence of the exogenous carbon source on the ex-situ bioremediation of a chronically PCB-contaminated soil Biotechnology and Bioengineering 64:240-249

·        Fava F., Di Gioia D., Marchetti L. (2000) Role of the reactor configuration in the biological detoxification of a dump site-polychlorobiphenyl-contaminated soil in lab slurry phase conditions. Applied Microbiology and Biotechnology 53: 243-248

·        Nocentini M., Pinelli D., Fava F. (2000) Bioremediation of a soil contaminated by hydrocarbon mixtures: the residual concentration problem. Chemosphere  41: 1115-1123

·        Fava F., Di Gioia D. (2001) Soya lecithin effects on the aerobic biodegradation of polychlorinated biphenyls in an artificially-contaminated soil. Biotechnology & Bioengineering  72:177-184

·        Villarante, N.R., Armenante P.M., Quibuyen, T.A.O., Fava F., Kafkewitz D. (2001) Dehalogenation of dichloroethene in a contaminated soil: fatty acids and alcohols as electron donors and an apparent requirement for tetrachloroethene. Applied Microbiology and Biotechnology, 55: 239-247

·        Di Gioia D., Bertin L., Fava F., Marchetti L. (2001) Biodegradation of hydroxylated and methoxylated benzoic, phenylacetic and phenylpropenoic acids present in olive mill wastewaters by two bacterial strains.  Research in Microbiology 152:83-93

·        Di Gioia D., Fava F., Bertin L., Marchetti L. (2001) Biodegradation of synthetic and natural occurring mixtures of mono-cyclic aromatic compounds present in olive mill wastewaters by two aerobic bacteria. Applied Microbiology and Biotechnology 55: 619-626.

·        Bertin L., Majone M., Di Gioia D., Fava F. (2001) An aerobic fixed-phase biofilm reactor system for the degradation of the low-molecular weight aromatic compounds occurring in the effluents of anaerobic digestors treating olive mill wastewaters.  Journal of Biotechnology 87:161-177.

·        Fedi S., Carnevali M., Fava F., Andracchio A., Zappoli S., Zannoni D. (2001) Biodegradative activities and hybridization analysis of fifteen aerobic bacterial strains isolated from a chronically polychlorinated biphenyl (PCB) contaminated site. Research in Microbiology 152:583-592

·        Fava F., Piccolo A. (2002) Effects of humic substances on the bioavailability and aerobic biodegradation of polychlorinated biphenyls in a model soil.  Biotechnology & Bioengineering 77: 204-211

·        Fava F., Ciccotosto F.V. (2002) Effects of Randomly Methylated-b-Cyclodextrins (RAMEB) on the bioavailability and aerobic biodegradation of polychlorinated biphenyls in three pristine soils spiked with a transformer oil. Applied Microbiology and Biotechnology 58: 393-399

·        Demnerova, K., Fava F., Mackova M., Pazlarova J., Vosahlikova M., Novakova H., Jindrova E., Ryslava E., Macek T., Vrchotova N., Brenner V., Pavlu L., Totevova S., Kristoffer T., Focht D.D., Di Gioia D., Marchetti L, Fletcher J.S., Leigh M.B., Kucerova P., Stiborova H., Mateju V., Sobotka M., Kastanek F., Kastanek P., Kasak L. PCB-Approaches to possible removal from the environment. (Book Chapter) In press in: "Innovative Approaches to the On-Site Assessment and Remediation of Contaminated Sites". NATO Advanced Study Institute book

·        Di Gioia D., Barberio C, Spagnesi S., Marchetti L., Fava F. (2002) Characterisation of four olive-mill wastewater indigenous bacteria capable of aerobically degrading hydroxylated- and methoxylated-monocyclic aromatic compounds. Archives of Microbiology 178: 208-217

·        Zanaroli G., Fedi S, Carnevali M, Fava F, Zannoni D. (2002) Use of potassium tellurite for testing survival and viability of Pseudomonas pseudoalcaligenes KF707 in soil microcosms contaminated with polychlorinated biphenyls (PCBs). Research in Microbiology 153:353-360.