Role of CycloLab: Coordinator
Integrated methodology for characterization of soil bioremediation (BIO-00066/2000)
Duration: 2000-2003
INTEGRATED METHODOLOGY FOR MONITORING OF IN SITU BIOREMEDIATION OF SOILS CONTAMINATED WITH POORLY BIODEGRADABLE OIL AND HEAVY METALS
The project has been completed. The novelty of the project is that the physico-chemical, biological and ecotoxicological methods used together in appropriate combinations have been integrated into a “Soil Testing Triad” to get appropriate data for decisions, planning, controlling and monitoring of bioremediation of sites with aged contamination. Such an integrated methodology able to monitor the processes in the soil during bioremediation has not been known so far.
1. Method development, compilation of the integrated methodology
For determination of total petroleum hydrocarbons, PAH content, volatile chlorinated organic components and polychlorinated biphenyls (PCBs) chromatographic methods were used. The toxic metal content of the soils has been quantified by ICP-MS technique. A new HPLC method has been developed for measuring the changes of the concentration of the cyclodextrin applied as an additive in bioremediation of contaminated soils.
Beside the standard methods for measuring the soil pH, exchangable cations, organic content, redox properties new methods for characterization of the transport processes (real permeability and capillary water movement) have been developed.
A lot of standard biological and ecotoxicological methods for ground water and soil extracts have been adapted to soil. New contact bio-tests have been developed using bacterial, plant and animal test organisms. A novelty is the application of the nitrification activity of the soils in ecotoxicological tests. The determination of the total ATP content of the soil is new, too. Microcosm tests have been worked out to follow the processes of bioremediation.
2. Application of the methodology for laboratory biodegradation experiments on soils contaminated with organic pollutants and inorganic microcontaminants
A number of laboratory experiments have been performed to test the effects of cyclodextrins in various concentrations, temperature, nutrients and inoculation to select the methods especially useful and informative on the actual site and actual contaminants taking into consideration the biodegradability, mobility, bioavailability of the pollutants, microbial activity, effects of additives, metabolites and the residual contamination.
3. Technology experiments and using the methodology to follow them
Based on the results of the laboratory scale technological experiments pilot experiments have been performed to study the applicability of a newly developed technology and select the most appropriate procedures. The integrated methodology was tested, too.
4. Planning and application of the new technologies
During the step-by-step scaling up field experiments have been performed using the new integrated methodology for the assessment of the site, planning, monitoring and controlling of the selected technology. The new methodology was applied also for the assessment of the secondary environmental and human risks caused by the technology, for instance, for the estimation of the risk exerted by the changes in the mobility of the pollutants on the effect of the bioavailability-increasing additives and the toxic intermediates of the biochemical processes in case of in situ technologies.
5. Dissemination of the results
The results have been published in 5 scientific papers and over 20 presentations at national and international conferences.
COORDINATOR
Prof. Dr. József SZEJTLI
CycloLab Cyclodextrin R&D Laboratory Ltd.
1097 Budapest Illatos 7.
Mail: 1525 Budapest P.O. Box 435
Tel.: 347-6060
Fax: 347-6068
e-mail: cyclolab@cyclolab.hu
SUBCONTRACTORS
Dr. Attila MURÁNYI
Environment Quality Trade and Service Co.
1027 Budapest, Bem rkp. 54.
Tel./Fax: 361-316-3178
e-mail: attila.muranyi@rissac.hu
Dr. Mária BÁLINT
Bálint Analytics Ltd.
1071 Budapest Damjanich 33.
Tel./Fax: 361-206-0732
e-mail: balintanal@matavnet.hu
PARTNERS
Dr. Katalin GRUIZ
Budapest University of Technology and Economics, Department of Agricultural Chemical Technology
1111 Budapest, Gellért sq. 4.
Tel.: 361-463-2347 Fax:361-463-2598
E-mail: gruiz@mail.bme.hu
Edward G. SOMÉUS
TDT-3R Ltd.
1222 Budapest, Széchenyi 59.
Tel./Fax: 361-424-0466
e-mail: edward@inext.hu
|
